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Guidance lecture of malaysia matriculation biology, experiment 3 : plant histology.
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Lab report 6 Biology SB015
Biologi (sb02), kolej matrikulasi perak.
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Experiment 6: isolating dna.
Objectives: At the end of this lesson, students should be able to isolate DNA from plant tissue.
Materials: Ice-chilled 95% alcohol Ice cubes Kiwi/ banana/onion/tomato/watermelon 0 g ethylenediaminetetraacetic acid (EDTA) 50 g sodium dodecyl sulphate or sodium lauryl sulphate (SDS or SLS) 8 g sodium chloride 4 g sodium citrate 1 litre water
Apparatus: 500 ml beaker Boiling tube Boiling tube rack Mortar and pestle Muslin cloth Water bath (60°C)
Procedures and Observation:
1. the salt-detergent solution is prepared. to completely dissolve the, salt without producing foam stir gently., 2 ml of ice-chilled alcohol is poured into a boiling tube and it is, placed into a beaker containing ice cubes., 3. kiwi/ onion/ tomato/ banana/ watermelon is peel, sliced and mash, with mortar., 4 fruit is transfered into a beaker and 100 ml of the salt-, detergent solution is added. the mixture is incubated in the water, bath at 60oc for 15 minutes., 5. the mixture is sieved after 15 minutes, with muslin cloth and the, liquid in a beaker is collected., 6. the boiling tube is filled half with sieved liquid., 7. 10ml of ice-chilled alcohol is poured very carefully into the side of, the boiling tube at a flat angle., 8 boiling tube is put into a rack and observed. observe and draw, the extracted dna between alcohol and the sieved liquid is observed, and drawn. crude dna has been found in between the alcohol and, sieved liquid., dna isolating is the crucial step in the identification of, dna procedure consists of lysis or the breakdown of cell, wall or membrane to release the dna using salt-detergent, in this experiment,the fuits that we use is banana,onion,and, tomato. this experiment we rupture fruits cells, thus, releasing their contents such as protein, dna, rna, lipids,, ribosomes and various small molecules. dna is then, suspended by alcohol as supernatant layer that we can see in, the boiling tube that contain different extract of plant, which, is banana,onion and tomato observation that we, got,dna of banana is more clear than tomato and onion, because banana is has soft texture that not need to mash, harder than tomato and onion. we assume that dna, structure of tomato and onion is has been damage while we, mash them in mortar., the precaution step that can be taken is make sure both, liquids do not mix and alcohol form a separate layer on top of, the sieved liquid should not mash the fruits harder to, avoid the dna is damaged..
Conclusion Plant tissue can be isolate by breakdown of cell wall or membrane to release the DNA using salt-detergent solution to remove proteins that are bound to the DNA. It also helps to keep the proteins dissolved in the aqueous layer so they don't precipitate in the alcohol along with the DNA.
1. What is the purpose of using the following?
A. salt-detergent solution, remove proteins that are bound to the dna keep the, proteins dissolved in the aqueous layer so they don't, precipitate in the alcohol along with the dna., b. ice chilled alcohol, helps the dna to precipitate more quickly., c. water bath at 60oc, to increase the rate of reactions to unzip the double helix, to separate the dna from the proteins to cause the dna to, precipitate out to cut the dna into fragments., 2 do we need to mash the fruits, exposes a greater surface area from which to extract the dna..
- Multiple Choice
Course : biologi (SB02)
University : kolej matrikulasi perak.
- More from: biologi SB02 Kolej Matrikulasi Perak 51 Documents Go to course
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TITLE EXPERIMENT 2: PLANT TISSUES. OBJECTIVES-To identify different types of plant tissues -To compare the structure and distribution of tissues in monocots and dicots. APPARATUS AND MATERIALS. Compound light microscope, prepared slides of cross sections of monocot stem and root, prepared slides of cross sections of dicot stem and roots.
labuan matriculation college batch 2022/ biology sb lab e-report experiment 2: plant tissues. course learning outcome (clo): name: putri erra elyana binti sharizall matric number: ms tutorial class: h lecturer's name: aini alira bt mohd adnan title : plant tissues date : 22/09/ objectives : i. to prepare onion epidermal cell slides ii.
Matriculation Lab Report : lab report biology sem 1(Experiment 2: Plant Histology) on August 19, 2020 Get link; Facebook; X; Pinterest; Email; Other Apps; Get link; ... Pre-lab/Lab report Chemistry Matrikulasi SEM1 (Chemical Equilibrium & Charles' Law ) Powered by Blogger Theme images by fpm.
Matriculation Division, Ministry of Education Malaysia. Published in Malaysia by ... Record only what you observe in the experiment. x Laboratory Report and Evaluation 1. Report should contain the following:-Title ... SB015 Lab Manual EXPERIMENT 2: PLANT TISSUES Course Learning Objective: Conduct biology laboratory work on
Quiz: E-LAB Report Plant Tissue Biology Matriculation Share. Created with AI from the Document. uploaded by. Anonymous Student. Question 1 of 10. Report. Question 1 of 10. Question. ... What is the purpose of staining onion cells with iodine solution during the experiment? A To kill the cells for observation. B To remove excess water from the ...
Lab report biology matriculation experiment 2 plant tissues When talking about the features of the tissues of plants, one has to keep in mind their evolutionary history. In the middle paleozoic period, about 450 to 500 million years ago, plants began to colonize the land, where the environment offered some advantages over the aquatic ...
Lab report biology matriculation experiment 2 plant tissues Plant tissue is a collection of similar cells performing an organized function for the plant. Each plant tissue is specialized for a unique purpose, and can be combined with other tissues to create organs such as leaves, flowers, stems and roots.
by Amran Md Said Matriculation College of Pahang. Experiment 3 : Plant Histology Objective 1. to prepare and identify the onion epidermal cells 2. to identify different types of plant tissues 3. to compare the structure and distribution of tissues in monocots and dicots Exercise 3.1 : Identification of epidermal cells in onion.
Example lab report biology matriculation experiment 1. Lab report example biology pdf matriculation experiment 1. Plant Histology on August 19 2020. 1 Writing a Biology Lab Report A special thanks goes to Katie Jones the author of our biology lab report samples. Lab Report Rubric - Lab Report Rubric Login or Sign Up.
LAB Report BIO EXP 7 - experiment 7 of biology course; Assignments SB02 5 - About biodiversity ... Conclusion Plant tissue can be isolate by breakdown of cell wall or membrane to release the DNA using salt-detergent solution to remove proteins that are bound to the DNA. It also helps to keep the proteins dissolved in the aqueous layer so they ...